Bicd2^tm1.1Hgrd
Targeted Allele Detail

Summary

• Symbol: Bicd2^tm1.1Hgrd
• Name: BICD cargo adaptor 2; targeted mutation 1.1, Casper Hoogenraad
• MGI ID: MGI:5618154
• Synonyms: Bicd2^-
• Gene: Bicd2 Location: Chr13:49495061-49540502 bp, + strand Genetic Position: Chr13, 25.22 cM
• Alliance: Bicd2^tm1.1Hgrd page

Hydrocephalus, enlarged lateral ventricles, thinning of the cortex, and atrophy of several telencephalic areas in Bicd2^tm1.1Hgrd/Bicd2^tm1.1Hgrd mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210236
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: The endogenous locus was targeted with two rounds of homologous recombination that inserted a floxed neomycin selection cassette into intron 1 and a cassette containing a floxed puromycin resistance gene followed by the lacZ reporter gene fused to a nuclear localization signal (nls-lacZ) into the 3' untranslated region. Exons 2 through 9 were flanked by the floxed selection genes. Cre meditated recombination removed both selection cassettes and exons 2-9. Western blot analysis confirmed the absence of protein in brain lysates of P20 homozygous mutant mice. (J:210236)

Generation of the Bicd2^tm1Hgrd and Bicd2^tm1.1Hgrd alleles

Expression

In Structures Affected by this Mutation:

14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bicd2 Mutation: 31 strains or lines available

References

• Original: J:210236 Jaarsma D, et al., A role for Bicaudal-D2 in radial cerebellar granule cell migration. Nat Commun. 2014;5:3411
• All: 1 reference(s)