Igk^{tm3.1(IGK)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Igk^{tm3.1(IGK)Vlcg}
• Name: immunoglobulin kappa chain complex; targeted mutation 3.1, Velocigene
• MGI ID: MGI:5610005
• Synonyms: 40hVK, 40hVKdHyg
• Gene: Igk Location: Chr6:67532620-70703738 bp, + strand Genetic Position: Chr6, 30.89 cM, cytoband F1-pter

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208619
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Igk^{tm3.1(IGK)Vlcg} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  human	IGK (50802)

• Mutation details: Using BAC recombineering roughly 3 Mbp of the mouse locus including all of the Vk and Jk segments was replaced with 480 kb of human IGK sequence including the proximal Vk and all of the Jk human gene segments. The final selection cassette was removed by flp-mediated recombination. (J:208619)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igk Mutation: 25 strains or lines available

References

• Original: J:208619 Macdonald LE, et al., Precise and in situ genetic humanization of 6 Mb of mouse immunoglobulin genes. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5147-52
• All: 3 reference(s)