Prdm9^{tm1(PRDM9)Wthg}
Targeted Allele Detail

Summary

• Symbol: Prdm9^{tm1(PRDM9)Wthg}
• Name: PR domain containing 9; targeted mutation 1, Benjamin Davies
• MGI ID: MGI:5609598
• Gene: Prdm9 Location: Chr17:15763341-15784616 bp, - strand Genetic Position: Chr17, 8.95 cM
• Alliance: Prdm9^{tm1(PRDM9)Wthg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228712
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Humanized sequence, RMCE-ready)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The region of exon 10 of the endogenous mouse gene that encodes the zinc-finger array has been replaced by the sequence of the corresponding region of human PRDM9 (reference allele, variant B); coding DNA upstream of the first zinc finger and the sequences downstream of the translation stop codon are of mouse origin. Upstream and downstream, respectively, of exon 10 reside a single PhiC31 attP site and a frt site-flanked selection cassette with a second attP site between its PGK promoter and neomycin phosphotransferase coding sequence; the attP sites allow for PhiC31 integrase-mediated cassette exchange. (J:228712)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm9 Mutation: 45 strains or lines available

References

• Original: J:228712 Davies B, et al., Re-engineering the zinc fingers of PRDM9 reverses hybrid sterility in mice. Nature. 2016 Feb 11;530(7589):171-6
• All: 1 reference(s)