Tg(CAG-RFP,Acr-EGFP)RBGS002Osb
Transgene Detail

Summary

• Symbol: Tg(CAG-RFP,Acr-EGFP)RBGS002Osb
• Name: transgene insertion RBGS002, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:5607931
• Synonyms: Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
• Transgene: Tg(CAG-RFP,Acr-EGFP)RBGS002Osb Location: unknown
• Alliance: Tg(CAG-RFP,Acr-EGFP)RBGS002Osb page

Transgene origin

• Strain of Origin: C57BL/6 x DBA/2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: Two transgenic constructs were coinjected. The first construct has the CAG promoter fused to a mitochondrial import sequence (Atp5g1) driving expression of RFP (DsRed2). The second construct has the acrosin promoter with a proacrosin signal sequence driving expression of GFP. The sperm head is green prior to the acrosome reaction, after acrosome reaction GFP expression is lost. RFP expression is seen in the mitochondria in the midpiece of the sperm. (J:216056)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:216056 Hasuwa H, et al., Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo. Exp Anim. 2010;59(1):105-7
• All: 37 reference(s)