Tshz1^tm1Garr
Targeted Allele Detail

Summary

• Symbol: Tshz1^tm1Garr
• Name: teashirt zinc finger family member 1; targeted mutation 1, Alistair N Garratt
• MGI ID: MGI:5588813
• Synonyms: Tshz1^GFP
• Gene: Tshz1 Location: Chr18:84029752-84105831 bp, - strand Genetic Position: Chr18, 57.53 cM
• Alliance: Tshz1^tm1Garr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209428
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A GAP43-EGFP cassette and a self-excision cre-neomycin cassette (SEcre Neo) was fused in-frame following the first five codon of exon2 while removing most of the open reading frame of Tshz1 gene by homologous recombination. SEcre Neo cassette was removed in chimera male germline leaving fusion protein contains the first 18 amino acids of TSHZ1 (ending in YVPE encoded at the start of exon 2), followed by the N-terminal 23 amino acids of GAP43 and EGFP sequences, ending with the termination codon of EGFP. The in situ hybridization and immunostaining on homozygous embryos confirmed absence of transcripts and functional protein products. This is a null allele. (J:209428)

Expression

In Mice Carrying this Mutation:	192 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tshz1 Mutation: 51 strains or lines available

References

• Original: J:209428 Ragancokova D, et al., TSHZ1-dependent gene regulation is essential for olfactory bulb development and olfaction. J Clin Invest. 2014 Mar 3;124(3):1214-27
• All: 4 reference(s)