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Col1a1tm4(tetO-Jun/Fosl2)Wag
Targeted Allele Detail
Nomenclature
Symbol: Col1a1tm4(tetO-Jun/Fosl2)Wag
Name: collagen, type I, alpha 1; targeted mutation 4, Erwin F Wagner
MGI ID: MGI:5586533
Synonyms: Col1a1TetOP-c-Jun~Fosl2
Gene: Col1a1  Location: Chr11:94936224-94953042 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:210545
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
Mutation:    Insertion
 
Col1a1tm4(tetO-Jun/Fosl2)Wag expresses 2 genes
 
Mutation detailsKH2 ES cells were retargeted with a "flp-in plasmid" containing a tetracycline responsive element (TRE or tetO) and a forced dimerization fusion cDNA of Jun lacking the stop codon and Fosl2 connected by a flexible short polyGly-Ser peptide with a C-terminal FLAG-tag. A flpe-recombinase plasmid facilitated integration into the 3' UTR region. (J:210545)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  104 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm2(tetO-Pou5f1)Jae (J:159351).
References
Original:  J:210545 Hasenfuss SC, et al., Regulation of steatohepatitis and PPARgamma signaling by distinct AP-1 dimers. Cell Metab. 2014 Jan 7;19(1):84-95
All:  1 reference(s)

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last database update
10/10/2017
MGI 6.10
The Jackson Laboratory