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Slc1a2tm1.1Ncd
Targeted Allele Detail
Nomenclature
Symbol: Slc1a2tm1.1Ncd
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2; targeted mutation 1.1, NC Danbolt
MGI ID: MGI:5576462
Synonyms: EAAT2-flox
Gene: Slc1a2  Location: Chr2:102658659-102790784 bp, + strand  Genetic Position: Chr2, 54.13 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:207177
Parent Cell Line:  D1 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6J)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA loxP site was inserted into intron 2 and an frt-neo-frt-loxP cassette was inserted into intron 4, floxing exons 3 and 4 of the Slc1a2 gene from the bacterial artificial chromosome clone RP24-315H4. The neomycin cassette was removed via FLP-mediated recombination, leaving exons 3 and 4 floxed by loxP sites. Cre-mediated recombination can be used to remove exons 3 and 4 which results in a frameshift deletion. (J:207177)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Slc1a2 Mutation:  1 strain or line available
References
Original:  J:207177 Zhou Y, et al., Proteome analysis and conditional deletion of the EAAT2 glutamate transporter provide evidence against a role of EAAT2 in pancreatic insulin secretion in mice. J Biol Chem. 2014 Jan 17;289(3):1329-44
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/10/2019
MGI 6.14
The Jackson Laboratory