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Insm1tm1.1Mgn
Targeted Allele Detail
Nomenclature
Symbol: Insm1tm1.1Mgn
Name: insulinoma-associated 1; targeted mutation 1.1, Mark A Magnuson
MGI ID: MGI:5576020
Synonyms: Insm1(GFP-Cre)
Gene: Insm1  Location: Chr2:146221997-146225020 bp, + strand  Genetic Position: Chr2, 71.33 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:94077
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Recombinase, Reporter)
Mutation:    Insertion
 
Mutation detailsUsing recombinase mediated cassette exchange (RMCE) a 532 bp fragment containing a GFP-Cre fusion protein replaced the entire coding region of the gene. The donor construct includes a frt-flanked pgk-hygromycin immediately downstream of the GFP-Cre fusion sequence. The frt-flanked pgk-hygromycin was removed by FLPe mediated recombination. Western blot analysis confirmed absence of protein in homozygotes. Green fluorescence is seen in pancreatic pre-endocrine cells and in islet cells after birth, in the developing central nervous system between E9.5 and E18.5, and also in the peripheral nervous system and gut endocrine cells. (J:94077, J:214095)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Insm1     Summary of all recombinase alleles driven by Insm1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Insm1 Mutation:  4 strains or lines available
References
Original:  J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/30/2020
MGI 6.15
The Jackson Laboratory