Insm1tm1.1Mgn
Targeted Allele Detail
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Symbol: |
Insm1tm1.1Mgn |
Name: |
insulinoma-associated 1; targeted mutation 1.1, Mark A Magnuson |
MGI ID: |
MGI:5576020 |
Synonyms: |
Insm1(GFP-Cre) |
Gene: |
Insm1 Location: Chr2:146064021-146066940 bp, + strand Genetic Position: Chr2, 71.33 cM
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Alliance: |
Insm1tm1.1Mgn page
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Germline Transmission: |
Earliest citation of germline transmission:
J:94077
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Recombinase, Reporter) |
Mutation: |
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Insertion
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Insm1tm1.1Mgn expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: Using recombinase mediated cassette exchange (RMCE) a 532 bp fragment containing a GFP-Cre fusion protein replaced the entire coding region of the gene. The donor construct includes a frt-flanked pgk-hygromycin immediately downstream of the GFP-Cre fusion sequence. The frt-flanked pgk-hygromycin was removed by FLPe mediated recombination. Western blot analysis confirmed absence of protein in homozygotes. Green fluorescence is seen in pancreatic pre-endocrine cells and in islet cells after birth, in the developing central nervous system between E9.5 and E18.5, and also in the peripheral nervous system and gut endocrine cells.
(J:94077, J:214095)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Insm1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015; |
All: |
7 reference(s) |
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