Plg^tm1.1Fjc
Targeted Allele Detail

Summary

• Symbol: Plg^tm1.1Fjc
• Name: plasminogen; targeted mutation 1.1, Francis J Castellino
• MGI ID: MGI:5571896
• Synonyms: Plg^S743A
• Gene: Plg Location: Chr17:12597496-12638271 bp, + strand Genetic Position: Chr17, 8.5 cM
• Alliance: Plg^tm1.1Fjc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210802
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Nucleotide substitutions (AGT to GCC) within exon 19 result in the amino acid substitution of alanine for serine at position 743 (S743A). Flp-mediated recombination removed the loxP- and FRT-flanked neomycin resistance cassette inserted downstream of the modified exon 19. This results in a plasmin-inactivating active site mutation. Mice expression normal levels of protein in the plasma but no activity towards the Plm chromogenic substrate, S2251, despite formation of two-chain Plm. (J:210802)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plg Mutation: 4 strains or lines available

References

• Original: J:210802 Iwaki T, et al., The generation and characterization of mice expressing a plasmin-inactivating active site mutation. J Thromb Haemost. 2010 Oct;8(10):2341-4
• All: 5 reference(s)