Kit^{tm1.1(cre)Jmol}
Targeted Allele Detail

Summary

• Symbol: Kit^{tm1.1(cre)Jmol}
• Name: KIT proto-oncogene receptor tyrosine kinase; targeted mutation 1.1, Jeffery D Molkentin
• MGI ID: MGI:5571474
• Synonyms: Kit^Cre
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^{tm1.1(cre)Jmol} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210584
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutation: Insertion
• Kit^{tm1.1(cre)Jmol} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Kit (MGI:96677)

• Mutation details: A cDNA encoding a cre-IRES-nlsEGFP (cre recombinase separated by an internal ribosomal entry site from the nuclear localized EGFP coding sequence) cassette and an frt-flanked neomycin selection marker were cloned in-frame at the Kit ATG start site in exon 1. Homologous recombination was performed in ES cells which were aggregated with 8-cell embryos to generate chimeras. Chimeras were crossed to knock-in flp-expressing mice to delete the neo marker. Animals produce a bicistronic transcript for production of cre and EGFP proteins. (J:101977, J:210584)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Kit (mouse) Summary of all recombinase alleles driven by Kit. Comparative matrix view of recombinase activities Alliance mouse Kit gene page

Expression

In Mice Carrying this Mutation:

21 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 179 strains or lines available

References

• Original: J:210584 van Berlo JH, et al., c-kit+ cells minimally contribute cardiomyocytes to the heart. Nature. 2014 May 15;509(7500):337-41
• All: 7 reference(s)