Rho^tm1.1Eye
Targeted Allele Detail

Summary

• Symbol: Rho^tm1.1Eye
• Name: rhodopsin; targeted mutation 1.1, Stephen H Tsang
• MGI ID: MGI:5566834
• Synonyms: Rho^D190N
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^tm1.1Eye page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205477
• Parent Cell Line: KV1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: A G to A point mutation, converting the 190th amino acid of Rho from aspartic acid (D) to asparagine (N), was introduced using the GalK pop-in/pop-out recombineering method on RP24-288F10 BAC from BACPAC Resource. A loxP-Neo-loxp cassette that was inserted in the intron downstream of the exon carrying the D190N mutation was removed via cre-mediated recombination. (J:205477)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 48 strains or lines available

References

• Original: J:205477 Sancho-Pelluz J, et al., Mice with a D190N mutation in the gene encoding rhodopsin: a model for human autosomal-dominant retinitis pigmentosa. Mol Med. 2012;18:549-55
• All: 2 reference(s)