Flt3^tm2.1Dosm
Targeted Allele Detail

Summary

• Symbol: Flt3^tm2.1Dosm
• Name: FMS-like tyrosine kinase 3; targeted mutation 2.1, Donald Small
• MGI ID: MGI:5565506
• Synonyms: FLT3/D835Y
• Gene: Flt3 Location: Chr5:147267551-147337299 bp, - strand Genetic Position: Chr5, 86.88 cM, cytoband G3
• Alliance: Flt3^tm2.1Dosm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205200
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutations: Insertion, Single point mutation
• Mutation details: An inverted floxed pgk-neo cassette was inserted in intron 19 and codon 838 was changed to TAC from TAT in exon 20 via homologous recombination. This results in a D838Y mutation corresponding to the D835Y mutation seen in patients with acute myeloid leukemia. Cre mediated recombination removed the neo cassette. (J:205200)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Flt3 Mutation: 83 strains or lines available

References

• Original: J:205200 Bailey E, et al., FLT3/D835Y mutation knock-in mice display less aggressive disease compared with FLT3/internal tandem duplication (ITD) mice. Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):21113-8
• All: 1 reference(s)