Tg(INS-HBEGF*L148S*P149T)70Rin
Transgene Detail

Summary

• Symbol: Tg(INS-HBEGF*L148S*P149T)70Rin
• Name: transgene insertion 70, The Tokyo Metropolitan Institute of Medical Science
• MGI ID: MGI:5565218
• Synonyms: INS-TRECK-Tg, Tg(INS-TRECK/DTR)1Rin
• Transgene: Tg(INS-HBEGF*L148S*P149T)70Rin Location: unknown
• Alliance: Tg(INS-HBEGF*L148S*P149T)70Rin page

Transgene origin

• Strain of Origin: C.BKa-Prkdc^scid/IcrJcl

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(INS-HBEGF*L148S*P149T)70Rin expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

  Transgene codes for a protein with leucine to serine and proline to threonine mutations.

• Mutation details: This transgene consists of the 1.9 kb human insulin promoter region, rabbit beta-globin intron, human diphtheria toxin receptor (HBEGF) with L148S/P149T mutations, and the polyadenylation signals of rabbit beta-globin and SV40. It was injected into the pronucleus of Prkdc^scid eggs. Transgene expression is seen in pancreatic beta cells and administration of diphtheria toxin results in HBEGF expressing cells. (J:204420)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:204420 Matsuoka K, et al., Generation of mouse models for type 1 diabetes by selective depletion of pancreatic beta cells using toxin receptor-mediated cell knockout. Biochem Biophys Res Commun. 2013 Jul 5;436(3):400-5
• All: 2 reference(s)