Gtf2a1^tm1.1Jjdh
Targeted Allele Detail

Summary

• Symbol: Gtf2a1^tm1.1Jjdh
• Name: general transcription factor II A, 1; targeted mutation 1.1, James J Hsieh
• MGI ID: MGI:5565081
• Synonyms: TFIIAalpha-beta^nc
• Gene: Gtf2a1 Location: Chr12:91522036-91557261 bp, - strand Genetic Position: Chr12, 45.02 cM, cytoband E
• Alliance: Gtf2a1^tm1.1Jjdh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205038
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site was inserted upstream of exon 6, the D/G of the Taspase 1 cleavage site in exon 6 were mutated to A/A, and a floxed pgk-neo cassette was inserted downstream of exon 7. Cre mediated recombination removed the neo cassette. Expression of the noncleavable form is increased compared to the processed product. (J:205038)

Expression

In Mice Carrying this Mutation:	15 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gtf2a1 Mutation: 63 strains or lines available

References

• Original: J:205038 Oyama T, et al., Cleavage of TFIIA by Taspase1 activates TRF2-specified mammalian male germ cell programs. Dev Cell. 2013 Oct 28;27(2):188-200
• All: 3 reference(s)