Crb3^tm1.2Bmar
Targeted Allele Detail

Summary

• Symbol: Crb3^tm1.2Bmar
• Name: crumbs family member 3; targeted mutation 1.2, Ben Margolis
• MGI ID: MGI:5563835
• Gene: Crb3 Location: Chr17:57368583-57372920 bp, + strand Genetic Position: Chr17, 29.65 cM
• Alliance: Crb3^tm1.2Bmar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206750
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin resistance cassette was inserted downstream of exon 2. Exon 3 was replaced with a floxed exon 3-GFP fusion. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed the modified exon 3. Western blot analysis confirmed the absence of protein expression in E18.5 lungs and kidneys. (J:206750)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crb3 Mutation: 9 strains or lines available

References

• Original: J:206750 Whiteman EL, et al., Crumbs3 is essential for proper epithelial development and viability. Mol Cell Biol. 2014 Jan;34(1):43-56
• All: 1 reference(s)