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Targeted Allele Detail
Symbol: Mylktm1.1Bph
Name: myosin, light polypeptide kinase; targeted mutation 1.1, B Paul Herring
MGI ID: MGI:5563563
Synonyms: CArGf, MLCKf
Gene: Mylk  Location: Chr16:34745210-35002420 bp, + strand  Genetic Position: Chr16, 24.49 cM
Shorter small intestines in mgi:5563563/mgi:5563563 mgi:2653286/0 and mgi:5563563/mgi:5563564 mgi:2653286/0 mice

Show the 2 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:204964
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6N
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
Mutation detailsA loxP site was inserted upstream of the CArG (CC(A/T)6GG) regulatory element in exon 15. An FRT- and loxP-flanked neomycin resistance cassette was inserted downstream of the CArG element. Flp-mediated recombination removed the selection cassette and left the CArG element floxed. This allele targets the smMLCK isoform without affecting full-length Mylk or telokin. Western blot analysis confirmed normal expression of smMLCK isoform. (J:204964)
Generation of the Mylktm1.1Bph and Mylktm1.2Bph alleles
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mylk Mutation:  5 strains or lines available
Original:  J:204964 Chen M, et al., Regulation of 130-kDa smooth muscle myosin light chain kinase expression by an intronic CArG element. J Biol Chem. 2013 Nov 29;288(48):34647-57
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory