Tgfb1^{tm1(Tgfb3)Kul}
Targeted Allele Detail

Summary

• Symbol: Tgfb1^{tm1(Tgfb3)Kul}
• Name: transforming growth factor, beta 1; targeted mutation 1, Ashok B Kulkarni
• MGI ID: MGI:5559039
• Synonyms: TGF-beta1^Lbeta3
• Gene: Tgfb1 Location: Chr7:25386427-25404502 bp, + strand Genetic Position: Chr7, 13.98 cM
• Alliance: Tgfb1^{tm1(Tgfb3)Kul} page

Reduced size, kyphosis, tooth abnormalities, and decreased mineral density and dental attrition in Tgfb1^{tm1(Tgfb3)Kul}/Tgfb1^{tm1(Tgfb3)Kul} mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204892
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted Mutation: Insertion Mutation details: The HA-tagged coding sequence for Tgfb3 fused with the sequence of the endogenous gene's latency associated peptide (LAP) and followed by a floxed neomycin resistance cassette replaced exon 2. ELISA confirmed the absence of protein expression in the serum. Western blot analysis confirmed the expression of the HA-tagged protein. (J:204892)

Generation of the Tgfb1^tm1(Tgfb3)Kul allele

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tgfb1 Mutation: 34 strains or lines available

References

• Original: J:204892 Hall BE, et al., Transforming growth factor-beta3 (TGF-beta3) knock-in ameliorates inflammation due to TGF-beta1 deficiency while promoting glucose tolerance. J Biol Chem. 2013 Nov 1;288(44):32074-92
• All: 1 reference(s)