Col1a1^{tm1(tetO-Fos)Wag}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-Fos)Wag}
• Name: collagen, type I, alpha 1; targeted mutation 1, Erwin F Wagner
• MGI ID: MGI:5555845
• Synonyms: Col1a1^TetOP-c-Fos, Col^TetO-Fos
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-Fos)Wag} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201145
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-Fos)Wag} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Fos (MGI:95574)

• Col1a1^{tm1(tetO-Fos)Wag} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: KH2 ES cells containing Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} were retargeted with a "flp-in plasmid" containing a tetracycline responsive element (TRE or tetO) and FLAG-tagged Fos cDNA sequence. A flpe-recombinase plasmid to facilitated integration into the 3' UTR region. (J:201145)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:201145 Briso EM, et al., Inflammation-mediated skin tumorigenesis induced by epidermal c-Fos. Genes Dev. 2013 Sep 15;27(18):1959-73
• All: 3 reference(s)