Myod1^{tm1.1(cre/ERT,TVA)Gcg}
Targeted Allele Detail

Summary

• Symbol: Myod1^{tm1.1(cre/ERT,TVA)Gcg}
• Name: myogenic differentiation 1; targeted mutation 1.1, Gerard C Grosveld
• MGI ID: MGI:5547828
• Synonyms: MyoD^CE
• Gene: Myod1 Location: Chr7:46025898-46028516 bp, + strand Genetic Position: Chr7, 30.03 cM
• Alliance: Myod1^{tm1.1(cre/ERT,TVA)Gcg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205518
• Parent Cell Line: F12 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Myod1^{tm1.1(cre/ERT,TVA)Gcg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myod1 (MGI:97275)

• Mutation details: A targeting vector was designed to insert the sequence encoding cre/ERT (CreER), an IRES-TVA (avian leukosis virus receptor) element, a floxed tk-NEO selection cassette, and an IRES sequence into the ATG start codon of the Myod1 gene. The construct was electroporated into "129SvJ" derived F12 embryonic stem (ES) cells, which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone cre-mediated recombination and no longer retained the cassette were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. (J:205518)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Myod1 (mouse) Summary of all recombinase alleles driven by Myod1. Alliance mouse Myod1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myod1 Mutation: 23 strains or lines available

References

• Original: J:205518 Blum JM, et al., Distinct and overlapping sarcoma subtypes initiated from muscle stem and progenitor cells. Cell Rep. 2013 Nov 27;5(4):933-40
• All: 1 reference(s)