Cr2^tm2.1Jhws
Targeted Allele Detail

Summary

• Symbol: Cr2^tm2.1Jhws
• Name: complement receptor 2; targeted mutation 2.1, John Weiss
• MGI ID: MGI:5547397
• Gene: Cr2 Location: Chr1:194819119-194859024 bp, - strand Genetic Position: Chr1, 98.44 cM
• Alliance: Cr2^tm2.1Jhws page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205353
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A self-excising neomycin resistance cassette replaced most of exons 1 through 8, econding the first six SCR domains of isoform Cr1. The remaining sequence fuses the Cr2 promoter, transcription start site and the first coding sequence (with the signal sequence) to the Cr2-specific C-terminal exons. The final allele lack the neomycin resistance cassette. Western blot analysis confirmed the absence of Cr1 protein expression in spleen lysate. Expression of Cr2 is maintained. (J:205353)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cr2 Mutation: 84 strains or lines available

References

• Original: J:205353 Donius LR, et al., Optimal germinal center B cell activation and T-dependent antibody responses require expression of the mouse complement receptor Cr1. J Immunol. 2013 Jul 1;191(1):434-47
• All: 1 reference(s)