Prmt5^{tm2c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Prmt5^{tm2c(EUCOMM)Wtsi}
• Name: protein arginine N-methyltransferase 5; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5546597
• Synonyms: Prmt5^F
• Gene: Prmt5 Location: Chr14:54744639-54754927 bp, - strand Genetic Position: Chr14, 27.82 cM, cytoband C1
• Alliance: Prmt5^{tm2c(EUCOMM)Wtsi} page
• IMPC: Prmt5 gene page

Mutation origin

• Mutant Cell Line: EPD0160_2_A08
• Germline Transmission: Earliest citation of germline transmission: J:201152
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 54750459 of Chromosome 14 upstream of exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 54751393. The critical exon 7 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Flp mediated recombination removed the lacZ cassette creating a floxed allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:201152, J:226257)

Disease models

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prmt5 Mutation: 44 strains or lines available

Notes

Mice were generated from cell line EPD0160_2_A08 in J:201152

References

• Original: J:201152 Bezzi M, et al., Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery. Genes Dev. 2013 Sep 1;27(17):1903-16
• All: 27 reference(s)