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Targeted Allele Detail
Symbol: Prmt5tm2c(EUCOMM)Wtsi
Name: protein arginine N-methyltransferase 5; targeted mutation 2c, Wellcome Trust Sanger Institute
MGI ID: MGI:5546597
Synonyms: Prmt5F
Gene: Prmt5  Location: Chr14:54507182-54517470 bp, - strand  Genetic Position: Chr14, 27.82 cM, cytoband C1
Mutant Cell Line:  EPD0160_2_A08
Germline Transmission:  Earliest citation of germline transmission: J:201152
Parent Cell Line:  JM8A3 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready)
Mutation:    Insertion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 54513936 of Chromosome 14 upstream of exon 7 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 54513002. The critical exon 7 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Flp mediated recombination removed the lacZ cassette creating a floxed allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:201152, J:226257)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 9 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Prmt5 Mutation:  31 strains or lines available
Mice were generated from cell line EPD0160_2_A08 in J:201152
Original:  J:201152 Bezzi M, et al., Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery. Genes Dev. 2013 Sep 1;27(17):1903-16
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory