Tcra^tm1Mass
Targeted Allele Detail

Summary

• Symbol: Tcra^tm1Mass
• Name: T cell receptor alpha chain; targeted mutation 1, Marc Schmidt-Supprian
• MGI ID: MGI:5532653
• Synonyms: Valpha14i^StopF
• Gene: Tcra Location: Chr14:52665424-54461655 bp, + strand Genetic Position: Chr14, cytoband D1-D2

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201417
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: The targeting construct inserted between the Jalpha and C alpha exons four polyadenylation sequences, a 1.8 kb of upstream regulatory sequence, the Valpha14 promoter, a leader sequence, a floxed stop cassette, the sequence for the Valpha14 and Jalpha18 invariant (Valpha14i) arrangement expressed in most NKT cells and a 0.2 kb intronic sequence. This insertion abolishes TCRalpha expression. (J:201417)

Schematic representaion of the Tcra^tm1Mass allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcra Mutation: 98 strains or lines available

References

• Original: J:201417 Vahl JC, et al., NKT cell-TCR expression activates conventional T cells in vivo, but is largely dispensable for mature NKT cell biology. PLoS Biol. 2013 Jun;11(6):e1001589
• All: 3 reference(s)