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Tdrkhtm1.2Hfl
Targeted Allele Detail
Nomenclature
Symbol: Tdrkhtm1.2Hfl
Name: tudor and KH domain containing protein; targeted mutation 1.2, Haifan Lin
MGI ID: MGI:5521576
Gene: Tdrkh  Location: Chr3:94413273-94434668 bp, + strand  Genetic Position: Chr3, 40.62 cM, cytoband F2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:197673
Parent Cell Line:  D2 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6J)F1
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neomycin resistance cassette and left exons 2 through 4 floxed. Cre-mediated recombination removed exons 2 through 4. Western blot analysis confirmed the absence of protein expression in testis. (J:197673)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tdrkh Mutation:  14 strains or lines available
References
Original:  J:197673 Saxe JP, et al., Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline. EMBO J. 2013 Jul 3;32(13):1869-85
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/18/2020
MGI 6.14
The Jackson Laboratory