Gt(ROSA)26Sor^{tm4(H1/tetO-RNAi:Ezh2)Arte}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(H1/tetO-RNAi:Ezh2)Arte}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, TaconicArtemis
• MGI ID: MGI:5521554
• Synonyms: Ezh2-tetKDA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(H1/tetO-RNAi:Ezh2)Arte} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:202724
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Knockdown)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(H1/tetO-RNAi:Ezh2)Arte} involves 1 genes/genome features (Ezh2) View all
• Mutation details: A short hairpin RNA directed against exon 8 of Ezh2 was targeted into the locus. The shRNA sequence is under the regulation of the H1/tetO operator fusion promoter and a codon-optimized tet repressor (itetR) driven by the CAGGS promoter. (J:202724)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:202724 Michalak EM, et al., Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool. Stem Cells. 2013 Sep;31(9):1910-20
• All: 1 reference(s)