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Igs2tm1(ACTB-EGFP,-tdTomato)Zng
Targeted Allele Detail
Nomenclature
Symbol: Igs2tm1(ACTB-EGFP,-tdTomato)Zng
Name: intergenic site 2; targeted mutation 1, Hui Zong
MGI ID: MGI:5520969
Synonyms: GT11ML, MADM-ML
Gene: Igs2  Location: unknown  Genetic Position: Chr11, Syntenic
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:202665
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsThe "multiple lox" MADM-ML-11 targeting vector has a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "GT MADM-ML" cassette. The "GT MADM-ML" cassette is designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; nucleotide 1-273), a beta-globin intronic sequence (containing an frt-lox5171-lox2272-frt-loxP-Neo-loxP), the C-terminal portion of a red fluorescent protein (tdTomato; nucleotide 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. (J:101977, J:202665)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Igs2 Mutation:  28 strains or lines available
References
Original:  J:202665 Henner A, et al., MADM-ML, a Mouse Genetic Mosaic System with Increased Clonal Efficiency. PLoS One. 2013;8(10):e77672
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
07/29/2020
MGI 6.15
The Jackson Laboratory