Chuk^tm2Yhu
Targeted Allele Detail

Summary

• Symbol: Chuk^tm2Yhu
• Name: conserved helix-loop-helix ubiquitous kinase; targeted mutation 2, Yinling Hu
• MGI ID: MGI:5515790
• Synonyms: Ikkalpha^K44A, Ikkalpha^KA
• Gene: Chuk Location: Chr19:44061774-44095919 bp, - strand Genetic Position: Chr19, 36.71 cM
• Alliance: Chuk^tm2Yhu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201799
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with a modified exon 2 in which nucleotide substitutions (AAG to GCG) result in the amino acid substitution of alanine for lysine at position 44 (K44A). This mutation produces a kinase inactive protein. A floxed neomycin resistance cassette was inserted downstream of the modified exon 2. Western blot analysis confirmed reduced protein expression in splenocytes. (J:188639, J:201799)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chuk Mutation: 48 strains or lines available

References

• Original: J:201799 Zhu F, et al., IKKalpha shields 14-3-3sigma, a G(2)/M cell cycle checkpoint gene, from hypermethylation, preventing its silencing. Mol Cell. 2007 Jul 20;27(2):214-27
• All: 5 reference(s)