Tg(UBC-CCR5,-CD4)19Mnz
Transgene Detail

Summary

• Symbol: Tg(UBC-CCR5,-CD4)19Mnz
• Name: transgene insertion 19, Michel C Nussenzweig
• MGI ID: MGI:5509007
• Transgene: Tg(UBC-CCR5,-CD4)19Mnz Location: unknown
• Alliance: Tg(UBC-CCR5,-CD4)19Mnz page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(UBC-CCR5,-CD4)19Mnz expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CCR5 (1234)

  Ccr5	(MGI:107182)

  human

  CD4 (920)

  Cd4	(MGI:88335)

• Mutation details: A transgene composed of human CCR5 and CD4 on a single poly-protein transcript separated by a ribosomal skip 2A was placed downstream of thehuman ubiquitin C promoter. A linearized 4.5 Kb fragment containing the transgene was injected into C57BL/6J fertilized female pronuclei. Founder line 19 was chosen based on surface expression of hCD4 and hCCR5 on peripheral blood mononuclear cells as determined by flow cytometry. (J:200013)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:200013 Gruell H, et al., Antibody and antiretroviral preexposure prophylaxis prevent cervicovaginal HIV-1 infection in a transgenic mouse model. J Virol. 2013 Aug;87(15):8535-44
• All: 1 reference(s)