Slc17a7^{tm1.1(cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Slc17a7^{tm1.1(cre)Hze}
• Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5507862
• Synonyms: Slc17a7-IRES2-Cre, Slc17a7-IRES2-Cre-Delta, Slc17a7^{m1(IRES2-Cre)Hze}, Vglut1-IRES2-Cre, Vglut1-IRES2-Cre-D, Vglut1-IRES2-Cre-Delta, Vglut1-IRES2-Cre-DeltaNeo/Hygro
• Gene: Slc17a7 Location: Chr7:44813373-44825566 bp, + strand Genetic Position: Chr7, 29.16 cM
• Alliance: Slc17a7^{tm1.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146821
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Slc17a7^{tm1.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc17a7 (MGI:1920211)

• Mutation details: The targeting vector contained, from 5' to 3', a partial Slc17a7 sequence spanning exon 1 through intron 9, an frt3 site within Slc17a7 intron 9, a partial Slc17a7 exon 10 sequence including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence , a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). The resulting Slc17a7-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice for several generations to remove PhiC31 transgene. (J:146821, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc17a7 (mouse) Summary of all recombinase alleles driven by Slc17a7. Alliance mouse Slc17a7 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Slc17a7 Mutation: 39 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 53 reference(s)