Rraga^tm1.1Dmsa
Targeted Allele Detail

Summary

• Symbol: Rraga^tm1.1Dmsa
• Name: Ras-related GTP binding A; targeted mutation 1.1, David Sabatini
• MGI ID: MGI:5499905
• Synonyms: RagA^GTP, RagA^Q66L
• Gene: Rraga Location: Chr4:86493910-86495522 bp, + strand Genetic Position: Chr4, 40.69 cM
• Alliance: Rraga^tm1.1Dmsa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194401
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129 and C57BL/6

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutations: Insertion, Single point mutation
• Mutation details: A floxed hygromycin resistance/STOP cassette was inserted upstream of a modified coding exon in which an A to T transversion results in the amino acid substitution of lysine for glutamine at position 66 (Q66L). Cre-mediated recombination removed the hygromycin resistance/STOP cassette. The predicted protein is constitutively active. (J:194401)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rraga Mutation: 16 strains or lines available

References

• Original: J:194401 Efeyan A, et al., Regulation of mTORC1 by the Rag GTPases is necessary for neonatal autophagy and survival. Nature. 2013 Jan 31;493(7434):679-83
• All: 5 reference(s)