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Rhotm4.1(RHO*/EGFP)Jhw
Targeted Allele Detail
Nomenclature
Symbol: Rhotm4.1(RHO*/EGFP)Jhw
Name: rhodopsin; targeted mutation 4.1, John H Wilson
MGI ID: MGI:5490865
Synonyms: P23H, P23H-hRho-GFP
Gene: Rho  Location: Chr6:115931748-115940036 bp, + strand  Genetic Position: Chr6, 53.72 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:196863
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Mutation
description
Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Rhotm4.1(RHO*/EGFP)Jhw expresses 1 gene
 
Mutation detailsThe endogenous gene was replaced with an HPRT minigene and a human RHO gene with a P23H mutation and a C terminal fusion to EGFP via homologous recombination. Cre mediated recombination removed the HPRT minigene. Expression levels of the fusion protein are reduced compared to wild-type protein expression and much of what is expressed is degraded. Most of the fusion protein is mislocalized to the inner segments and outer nuclear layer of the retina. (J:196863)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rho Mutation:  13 strains or lines available
References
Original:  J:196863 Price BA, et al., Mislocalization and degradation of human P23H-rhodopsin-GFP in a knockin mouse model of retinitis pigmentosa. Invest Ophthalmol Vis Sci. 2011 Dec;52(13):9728-36
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/24/2020
MGI 6.15
The Jackson Laboratory