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Targeted Allele Detail
Symbol: Rhotm4.1(RHO*/EGFP)Jhw
Name: rhodopsin; targeted mutation 4.1, John H Wilson
MGI ID: MGI:5490865
Synonyms: P23H, P23H-hRho-GFP
Gene: Rho  Location: Chr6:115908709-115916997 bp, + strand  Genetic Position: Chr6, 53.72 cM
Germline Transmission:  Earliest citation of germline transmission: J:196863
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Rhotm4.1(RHO*/EGFP)Jhw expresses 1 gene
Mutation detailsThe endogenous gene was replaced with an HPRT minigene and a human RHO gene with a P23H mutation and a C terminal fusion to EGFP via homologous recombination. Cre mediated recombination removed the HPRT minigene. Expression levels of the fusion protein are reduced compared to wild-type protein expression and much of what is expressed is degraded. Most of the fusion protein is mislocalized to the inner segments and outer nuclear layer of the retina. (J:196863)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rho Mutation:  37 strains or lines available
Original:  J:196863 Price BA, et al., Mislocalization and degradation of human P23H-rhodopsin-GFP in a knockin mouse model of retinitis pigmentosa. Invest Ophthalmol Vis Sci. 2011 Dec;52(13):9728-36
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.19
The Jackson Laboratory