H3f3b^tm1.2Mnn
Targeted Allele Detail

Summary

• Symbol: H3f3b^tm1.2Mnn
• Name: H3.3 histone B; targeted mutation 1.2, Jeff Mann
• MGI ID: MGI:5474378
• Gene: H3f3b Location: Chr11:115912738-115918788 bp, - strand Genetic Position: Chr11, 80.91 cM, cytoband E2
• Alliance: H3f3b^tm1.2Mnn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194069
• Parent Cell Line: 2A (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: A targeting vector was designed to contain, from 5' to 3', a synthetic splice acceptor, a loxP site, an ATG start codon, a wildtype H3 histone isoform 3 (H3.3) coding sequence, H3f3b (H3 histone, family 3B) 3' UTR, and an SV40 polyadenylation (polyA) sequence. This was followed by a downstream frt-flanked neomycin resistance (neo) cassette, and a second loxP site, an ATG start codon, the cerulean variant of cyan fluorescent protein (CFP), and another SV40 polyA sequence. This vector replaced all of exon 2. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed the H3.3 sequence and allowed for expression of the reporter gene. (J:194069)

Schematic of the H3f3a^tm1.1Mnn and H3f3b^tm1.2Mnn alleles

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H3f3b Mutation: 14 strains or lines available

References

• Original: J:194069 Tang MC, et al., Conditional allelic replacement applied to genes encoding the histone variant H3.3 in the mouse. Genesis. 2013 Feb;51(2):142-6
• All: 3 reference(s)