TgTn(sb-Tyr)2172Ove
Transgene Detail

Summary

• Symbol: TgTn(sb-Tyr)2172Ove
• Name: transgenic transposon concatemer 2172, Paul A Overbeek
• MGI ID: MGI:5473269
• Synonyms: OVB2172
• Transgene: TgTn(sb-Tyr)2172Ove Location: unknown
• Alliance: TgTn(sb-Tyr)2172Ove page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Transposon concatemer)
• Mutation: Insertion
• Mutation details: A Sleeping Beauty (SB) transposon transgenic approach was used to generate these mice. The pT2-BART3 transposon vector used here has (from 5' to 3') an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), an AD2 splice acceptor and polyA sequence, the tyrosinase minigene TyBS, a loxP site, a polyA sequence, a Hox9c splice acceptor, and right IR/DR (280 bp). The IR/DR sequences are outward-facing. The 4.1 kb tyrosinase minigene (TyBS) contains the tyrosinase upstream regulatory sequences (2.1 kbp from the BALB/c tyrosinase promoter and the first 65 bp of exon 1), followed by the C57BL/6-derived Tyr^s-J cDNA sequence (1.9 kbp including cysteine at amino acid 103, glycine at amino acid 346, and the stop codon with polyA signal). (J:192196)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:192196 Hicks AN, et al., Nicotinamide mononucleotide adenylyltransferase 2 (Nmnat2) regulates axon integrity in the mouse embryo. PLoS One. 2012;7(10):e47869
• All: 1 reference(s)