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Tg(Thy1-Brainbow3.1)3Jrs
Transgene Detail
Nomenclature
Symbol: Tg(Thy1-Brainbow3.1)3Jrs
Name: transgene insertion 3, Joshua R Sanes
MGI ID: MGI:5469425
Synonyms: Brainbow 3.1 line 3, TSOGK3
Transgene: Tg(Thy1-Brainbow3.1)3Jrs  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6J x CBA
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsThe Thy1-Brainbow 3.1 transgene was designed by Drs. Dawen Cai and Joshua R.Sanes (Harvard University) with the mouse Thy1 (thymus cell antigen 1, theta) regulatory elements surrounding four fluorescent protein (Yellow fluorescent protein (Phialidium sp); Orange fluorescent protein: Green fluorescent protein; Far-red fluorescent protein) sequences uniquely flanked with pairs of incompatible lox sites. Specifically, this Brainbow 3.1 coding region contained (from 5' to 3') a loxP site, lox2272 site, loxN site, non-fluorescent nuclear localized derivative of phi-YFP (phiNFPnls), polyA sequence, loxP site, farnesylated mOrange2, polyA sequence, lox2272 site, farnesylated EGFP, polyA sequence, loxN site, farnesylated mKate2, polyA sequence. Line 3 was generated. (J:101977, J:197821)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:197821 Tg(ACTFLPe)9205DymCai D, et al., Improved tools for the Brainbow toolbox. Nat Methods. 2013 May 5;10(6):540-7
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory