Cd247^tm2.1Lov
Targeted Allele Detail

Summary

• Symbol: Cd247^tm2.1Lov
• Name: CD247 antigen; targeted mutation 2.1, Paul E Love
• MGI ID: MGI:5468025
• Synonyms: TCRzeta^6F
• Gene: Cd247 Location: Chr1:165616250-165704846 bp, + strand Genetic Position: Chr1, 73.14 cM, cytoband G-H
• Alliance: Cd247^tm2.1Lov page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:192915
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Not Applicable) Mutation: Insertion Mutation details: Exons 3 through 8 were replaced with the corresponding wild-type sequence with a C-terminal FLAG tag (6Y-FLAG), an FRT-flanked neo cassette, a loxP site and the corresponding sequence in which the six immunoreceptor tyrosine-based activation motif (ITAM) tyorsines mutated to encode phenylalanine followed by a C-terminal Myc tag (6F-Myc). Cre-mediated recombination removed the 6Y-FLAG tag and neo cassette. The 6F-Myc tag remains intact. (J:192915)

Generation of the Cd247^tm2Lov and Cd247^tm2.1Lov alleles

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd247 Mutation: 11 strains or lines available

References

• Original: J:192915 Hwang S, et al., Reduced TCR signaling potential impairs negative selection but does not result in autoimmune disease. J Exp Med. 2012 Sep 24;209(10):1781-95
• All: 4 reference(s)