Il7^tm1.1Iku
Targeted Allele Detail

Summary

• Symbol: Il7^tm1.1Iku
• Name: interleukin 7; targeted mutation 1.1, Koichi Ikuta
• MGI ID: MGI:5461069
• Synonyms: Il-7^GFP
• Gene: Il7 Location: Chr3:7637088-7678820 bp, - strand Genetic Position: Chr3, 2.02 cM
• Alliance: Il7^tm1.1Iku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:189751
• Parent Cell Line: KY1.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Murine BAC clone RP23-446B12 was modified by the insertion of EGFP cDNA and a floxed neomycin resistance cassette after a translation initiation codon in exon 1. The 8 kb homologous recombination targeting vector was then recovered from the modified BAC and used to generate this allele. The selection cassette was then excised by transient cre expression. GFP expression was detected in thymic stromal cells. (J:189751)

Expression

In Mice Carrying this Mutation:

56 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il7 Mutation: 33 strains or lines available

References

• Original: J:189751 Hara T, et al., Identification of IL-7-producing cells in primary and secondary lymphoid organs using IL-7-GFP knock-in mice. J Immunol. 2012 Aug 15;189(4):1577-84
• All: 6 reference(s)