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Targeted Allele Detail
Symbol: Rpa1tm1b(KOMP)Wtsi
Name: replication protein A1; targeted mutation 1b, Wellcome Trust Sanger Institute
MGI ID: MGI:5446173
Gene: Rpa1  Location: Chr11:75298166-75348324 bp, - strand  Genetic Position: Chr11, 45.79 cM
Mutant Cell Line:  EPD0395_5_A02
Germline Transmission:  Earliest citation of germline transmission: J:188991
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 75316809 of Chromosome 11 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 75315863. The critical exon(s) were thus flanked by loxP sites, and subsequent cre expression then excised this critical sequence resulting in a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 5 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rpa1 Mutation:  33 strains or lines available
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
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