About   Help   FAQ
Tg(ACTA1-TPM3*M9R)4Hrd
Transgene Detail
Nomenclature
Symbol: Tg(ACTA1-TPM3*M9R)4Hrd
Name: transgene insertion 4, Edna Hardeman
MGI ID: MGI:5444208
Synonyms: HSA-alphaTmslow(Met9Arg), HSA-alphaTmslow(Met9Arg), HSA-aTmslow(Met9Arg), HSA-aTmslow(Met9Arg), HSA-TPM3*M9R, TPM3(Met9Arg)
Transgene: Tg(ACTA1-TPM3*M9R)4Hrd  Location: unknown  
Transgene
origin
Strain of Origin:  FVB/NJ
Transgene
description
Transgene Type:    Transgenic (Dominant negative, Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(ACTA1-TPM3*M9R)4Hrd expresses 1 gene
 
Mutation detailsFirst, the human tropomyosin 3 gene was used to isolate a full-length alphaTmslow cDNA sequence. Next, the sequence was modified by site-directed mutagenesis to have the ATG-to-AGG base pair substitution encoding a methionine-to-arginine substitution at codon 9 (M9R); resulting in the dominant-negative alphaTmslow(Met9Arg) mutation associated with human nemaline myopathy. The mutant cDNA sequence was placed downstream of a 2.2 kbp human alpha-skeletal actin (ACTA1) promoter sequence, and upstream of a 1022 bp cassette containing the SV40 small t antigen intron and 3' untranslated region. Lines 4, 9 and 14 were generated with high, low and moderate expression levels, respectively. (J:67596)
Phenotypes
Loading...
View phenotypes for all genotypes (concatenated display).
Disease models
Loading...
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:67596 Corbett MA, et al., A mutation in alpha-tropomyosin(slow) affects muscle strength, maturation and hypertrophy in a mouse model for nemaline myopathy. Hum Mol Genet. 2001 Feb 15;10(4):317-28
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
03/24/2015
MGI 5.21
The Jackson Laboratory