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Tg(Adora2a-Chrm3*,-mCherry)AD6Blr
Transgene Detail
Summary
Symbol: Tg(Adora2a-Chrm3*,-mCherry)AD6Blr
Name: transgene insertion AD6, Bryan L Roth
MGI ID: MGI:5440979
Synonyms: adora2A-rM3Ds, adora2A-rM3Ds-IRES-mCherry
Transgene: Tg(Adora2a-Chrm3*,-mCherry)AD6Blr  Location: unknown  
Alliance: Tg(Adora2a-Chrm3*,-mCherry)AD6Blr page
Transgene
origin
Strain of Origin:  (C57BL/6 x SJL/J)F1
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Adora2a-Chrm3*,-mCherry)AD6Blr expresses 1 gene
 
Mutation detailsThe RP24-238K3 BAC harboring the adenosine A2a receptor locus (Adora2a), a ~40 kbp sequence proximal to the Adora2a locus (including part of the Specc1l locus) and ~117 kbp of sequence distal to the Adora2a locus (including the Upb1 locus and most of the 1110038D17Rik locus). A construct containing the mutant G protein-coupled receptor (GPCR) sequence, rM3Ds, with N-terminal hemagglutinin tag, an internal ribosome entry site (IRES), and mCherry fluorescent protein cDNA sequence was inserted downstream of the ATG start site of the Adora2a locus on the BAC via homologous recombination/BAC recombineering. The rM3Ds sequence is a Galphas-coupled rat muscarinic 3 DREADD (designer receptor exclusively activated by designer drug), also referred to as R-s, rM3/turkey beta1AR chimer DREADD, Gs-DREADD, Gs-D, or D-s. To create the rM3Ds sequence, the wild-type rat muscarinic 3 receptor (Chrm3) sequence was first modified to replace the second and third intracellular loops with the corresponding turkey beta1-adrenergic receptor (ADRB1 or beta1AR) sequences. The second modification introduced, via site-directed mutagenesis, the Y148C and A238G amino acid substitutions that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). Line AD6 was generated. (J:191042)
Phenotypes
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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:191042 Farrell MS, et al., A Galphas DREADD mouse for selective modulation of cAMP production in striatopallidal neurons. Neuropsychopharmacology. 2013 Apr;38(5):854-62
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory