Pkhd1^tm2Cjwa
Targeted Allele Detail

Summary

• Symbol: Pkhd1^tm2Cjwa
• Name: polycystic kidney and hepatic disease 1; targeted mutation 2, Christopher J Ward
• MGI ID: MGI:5438324
• Synonyms: Pkhd1^LSL(-)
• Gene: Pkhd1 Location: Chr1:20128003-20688288 bp, - strand Genetic Position: Chr1, 6.27 cM, cytoband A2-A5
• Alliance: Pkhd1^tm2Cjwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187735
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: The targeting vector was designed with a loxP-STOP-loxP (LSL) cassette containing a puromycin acetyltransferase gene (pac) and 4 copies of the SV40 viral transcriptional termination sequence inserted into intron 2 and two SV5-Pk epitope tags (26 amino acids long) inserted in-frame into exon 3. The LSL cassette terminates all transcripts in intron 2 generating a null allele. Northern blotting and RT-PCR analysis confirmed the absence of mRNA. (J:187735)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pkhd1 Mutation: 222 strains or lines available

References

• Original: J:187735 Bakeberg JL, et al., Epitope-tagged Pkhd1 tracks the processing, secretion, and localization of fibrocystin. J Am Soc Nephrol. 2011 Dec;22(12):2266-77
• All: 4 reference(s)