Pik3ca^tm1Gilb
Targeted Allele Detail

Summary

• Symbol: Pik3ca^tm1Gilb
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; targeted mutation 1, Richard Gilbertson
• MGI ID: MGI:5438138
• Synonyms: Pik3ca^E545K, Pik3ca^loxE545K
• Gene: Pik3ca Location: Chr3:32451203-32520256 bp, + strand Genetic Position: Chr3, 15.7 cM, cytoband B
• Alliance: Pik3ca^tm1Gilb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186709
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Single point mutation
• Mutation details: A floxed puro/STOP cassette was inserted upstream of the exon containing the initiation codon. Exon 9 was replaced with one in which nucleotide substitution results in the amino acid substitution of lysine for glutamic acid at position 545 (E545K). Flp-mediated recombination removed the floxed neo cassette inserted into intron 9. Cre-mediated recombination is required to achieve expression of the knock-in allele. (J:186709)

Tumor Data

List all tumor models in MMHCdb carrying Pik3ca^tm1Gilb

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3ca Mutation: 62 strains or lines available

References

• Original: J:186709 Robinson G, et al., Novel mutations target distinct subgroups of medulloblastoma. Nature. 2012 Aug 2;488(7409):43-8
• All: 9 reference(s)