Opn1sw^tm1Pugh
Targeted Allele Detail

Summary

• Symbol: Opn1sw^tm1Pugh
• Name: opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan); targeted mutation 1, Edward Pugh
• MGI ID: MGI:5431111
• Synonyms: Opn1sw^Neo
• Gene: Opn1sw Location: Chr6:29376670-29380512 bp, - strand Genetic Position: Chr6, 12.36 cM
• Alliance: Opn1sw^tm1Pugh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186155
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 1 was replaced with one in which an A to T transition results in the amino acid substitution of tyrosine for phenylalanine at position 81 (F81Y). An FRT-flanked neo cassette was inserted downstream of exon 3. qRT-PCR confirmed a 1000-fold reduction in transcript expression. (J:186155)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Opn1sw Mutation: 22 strains or lines available

References

• Original: J:186155 Daniele LL, et al., A mouse M-opsin monochromat: retinal cone photoreceptors have increased M-opsin expression when S-opsin is knocked out. Vision Res. 2011 Feb 23;51(4):447-58
• All: 4 reference(s)