Del(6Ephb6-Trpv6)2Mfre
Targeted Allele Detail

Summary

• Symbol: Del(6Ephb6-Trpv6)2Mfre
• Name: deletion, Chr 6, Marc Freichel 2
• MGI ID: MGI:5430774
• Synonyms: Del2Mfre, Ephb6/Trpv6^tm2.1Mfre, Trpv6^-
• Gene: Del(6Ephb6-Trpv6)2Mfre Location: unknown Genetic Position: Chr6, Syntenic

Prostate abnormalities in MGI:5430775/MGI:5430775 mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185629
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intergenic deletion, Intragenic deletion Del(6Ephb6-Trpv6)2Mfre involves 2 genes/genome features (Ephb6, Trpv6) View all Mutation details: A loxP site was inserted upstream of a exon 13. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 15. Cre-mediated recombination removed exons 13 through 15 of Trpv6, exons 17 and 18 of Ephb6 and the neo cassette. Western blot analysis confirmed the absence of protein expression in the epididymis. (J:185629)

Generation of the Del(6Ephb6-Trpv6)2Mfre allele

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(6Ephb6-Trpv6)2Mfre Mutation: 0 strains or lines available

References

• Original: J:185629 Weissgerber P, et al., Excision of Trpv6 gene leads to severe defects in epididymal Ca2+ absorption and male fertility much like single D541A pore mutation. J Biol Chem. 2012 May 25;287(22):17930-41
• All: 1 reference(s)