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Apc2tm1Mno
Targeted Allele Detail
Nomenclature
Symbol: Apc2tm1Mno
Name: adenomatosis polyposis coli 2; targeted mutation 1, Masaharu Noda
MGI ID: MGI:5428211
Gene: Apc2  Location: Chr10:80295977-80318263 bp, + strand  Genetic Position: Chr10, 39.72 cM, cytoband B5-C2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:184856
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsA construct containing an eGFP gene and an inverted neo cassette was inserted in frame in exon 2 after the initiation codon via homologous recombination. RT-PCR and western blot analysis confirmed the absence of expression in the brains of homozygous mice. EGFP expression was detected by RT-PCR and live fluorescent imaging in the nervous system and liver. (J:184856)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 9 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Apc2 Mutation:  2 strains or lines available
References
Original:  J:184856 Shintani T, et al., Directional neuronal migration is impaired in mice lacking adenomatous polyposis coli 2. J Neurosci. 2012 May 9;32(19):6468-84
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/13/2019
MGI 6.14
The Jackson Laboratory