Nprl3^tm2.1Drhi
Targeted Allele Detail

Summary

• Symbol: Nprl3^tm2.1Drhi
• Name: nitrogen permease regulator-like 3; targeted mutation 2.1, Douglas R Higgs
• MGI ID: MGI:5426856
• Synonyms: deltaP6-R3^-
• Gene: Nprl3 Location: Chr11:32181963-32217707 bp, - strand Genetic Position: Chr11, 18.83 cM
• Alliance: Nprl3^tm2.1Drhi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184965
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph, Modified regulatory region)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Mutation details: In ES cells carrying the Nprl3 allele, Hba erythroid-specific enhancer R3 in Nprl3 intron 2 was replaced with a loxP site flanked neomycin resistance gene cassette. Subsequent cre-mediated recombination removed the neo cassette and the 12402 bp span between the loxP site in place of Nprl3 promoter P6 (Rr246493) and enhancer R3 (Rr351), which also serves as a promoter for Nprl3 transcripts with alternative, more downstream, 1st exons. The deletion also includes putative Hba enhancer Rm or DHS-12 (Rr352) in Nprl3 intron 1 and Nprl3 exons 1 and 2. RNA-FISH analysis indicates Nprl3 expression is eliminated in the brain but not in erythroid cells. Expression in erythroid cells from homozygous mice is about 20% that of wild-type, presumably from alternatively spliced transcripts with alternative 1st exons. No protein expression was detected in any tissue. (J:184965)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nprl3 Mutation: 31 strains or lines available

References

• Original: J:184965 Kowalczyk MS, et al., Intragenic enhancers act as alternative promoters. Mol Cell. 2012 Feb 24;45(4):447-58
• All: 2 reference(s)