Runx1tm2.1(cre/Esr1*)Ims
Targeted Allele Detail
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Symbol: |
Runx1tm2.1(cre/Esr1*)Ims |
Name: |
runt related transcription factor 1; targeted mutation 2.1, Igor M Samokhvalov |
MGI ID: |
MGI:5316015 |
Synonyms: |
Runx1SACRE |
Gene: |
Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
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Alliance: |
Runx1tm2.1(cre/Esr1*)Ims page
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Germline Transmission: |
Earliest citation of germline transmission:
J:182232
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Runx1tm2.1(cre/Esr1*)Ims expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Runx1 (MGI:99852) |
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Mutation details: A targeting vector was composed of the sequence for a tamoxifen-inducible cre recombinase (flanked by two mutated estrogen receptors, MerCreMer) with the Engrailed-2 splice acceptor (En2SA) sequence preceding the coding region and a transcription STOP sequence followed the cre/Esr1*. A floxed neo selection cassette followed the STOP. Insertion of the En2SA sequence prevents splicing of the STOP out and disrupted Runx1 expression from both the proximal P2 and distal P1 promoters. The neo was subsequently deleted from the allele.
(J:182232)
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Generation of the Runx1tm2.1(cre/Esr1*)Ims allele. |
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Runx1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Runx1 Mutation: |
35 strains or lines available
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Original: |
J:182232 Tanaka Y, et al., Early ontogenic origin of the hematopoietic stem cell lineage. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4515-20 |
All: |
2 reference(s) |
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