Runx1^{tm2.1(cre/Esr1*)Ims}
Targeted Allele Detail

Summary

• Symbol: Runx1^{tm2.1(cre/Esr1*)Ims}
• Name: runt related transcription factor 1; targeted mutation 2.1, Igor M Samokhvalov
• MGI ID: MGI:5316015
• Synonyms: Runx1^SACRE
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^{tm2.1(cre/Esr1*)Ims} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:182232
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Runx1^{tm2.1(cre/Esr1*)Ims} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Runx1 (MGI:99852)

• Mutation details: A targeting vector was composed of the sequence for a tamoxifen-inducible cre recombinase (flanked by two mutated estrogen receptors, MerCreMer) with the Engrailed-2 splice acceptor (En2SA) sequence preceding the coding region and a transcription STOP sequence followed the cre/Esr1*. A floxed neo selection cassette followed the STOP. Insertion of the En2SA sequence prevents splicing of the STOP out and disrupted Runx1 expression from both the proximal P2 and distal P1 promoters. The neo was subsequently deleted from the allele. (J:182232)

Generation of the Runx1^{tm2.1(cre/Esr1*)Ims} allele.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Runx1 (mouse) Summary of all recombinase alleles driven by Runx1. Comparative matrix view of recombinase activities Alliance mouse Runx1 gene page

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 35 strains or lines available

References

• Original: J:182232 Tanaka Y, et al., Early ontogenic origin of the hematopoietic stem cell lineage. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4515-20
• All: 2 reference(s)