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Tg(tetO-COX8A/PstI*)1Ctm
Transgene Detail
Nomenclature
Symbol: Tg(tetO-COX8A/PstI*)1Ctm
Name: transgene insertion 1, Carlos T Moraes
MGI ID: MGI:5316004
Synonyms: mito-PstI
Transgene: Tg(tetO-COX8A/PstI*)1Ctm  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6 x SJL)F1
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inducible, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(tetO-COX8A/PstI*)1Ctm expresses 1 gene
 
Mutation detailsThis transgene consists of a mammalianized version of the bacterial PstI gene positioned behind a 5' mitochondrial targeting sequence from human COX VIII (COX8a) gene under the control of the tetracycline response element (TRE) promoter. The intervening sequence 8 (IVS8) was introduced between the TRE and the mito-PstI coding sequence. Southern blots indicate reduced levels of mtDNA and accumulation of partially deleted mtDNA in mice mated with tetracycline-controlled transactivator protein expressing mice, indicating that the expressed protein is functional and induces double stranded breaks in mtDNA. Treatment with Dox prevents expression of the transgene. (J:145737)
Phenotypes
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Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:145737 Fukui H, et al., Mechanisms of formation and accumulation of mitochondrial DNA deletions in aging neurons. Hum Mol Genet. 2009 Mar 15;18(6):1028-36
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory