Cfap126^{tm1.1(icre)Heli}
Targeted Allele Detail

Summary

• Symbol: Cfap126^{tm1.1(icre)Heli}
• Name: cilia and flagella associated protein 126; targeted mutation 1.1, Heiko Lickert
• MGI ID: MGI:5308442
• Synonyms: 1700009P17Rik^{tm1.1(icre)Heli}, Fltp^T2AiCre
• Gene: Cfap126 Location: Chr1:170941487-170954536 bp, + strand Genetic Position: Chr1, 78.96 cM
• Alliance: Cfap126^{tm1.1(icre)Heli} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:180887
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Cfap126^{tm1.1(icre)Heli} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cfap126 (MGI:1922722)

• Mutation details: A vector was constructed to insert a strept/FLAG-tandem affinity tag (SF-TAG) followed by a T2A peptide sequence (from Thosea asigna virus) and the codon-improved cre recombinase (icre) sequence into exon 6 of the 1700009P17Rik gene (called Flattop; Fltp). An Frt-flanked PGK-neo sequence followed cre in the construct. The targeting vector was introduced into IDG3.2 ES cells for homologous recombination. Southern blotting showed that recombination occurred on the C57BL/6J allele. After germline transmission was established, mice were crossed to Flpe-expressing line to remove the neo cassette. (J:180887)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cfap126 (mouse) Summary of all recombinase alleles driven by Cfap126. Comparative matrix view of recombinase activities Alliance mouse Cfap126 gene page

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cfap126 Mutation: 15 strains or lines available

References

• Original: J:180887 Lange A, et al., Fltp(T2AiCre): A new knock-in mouse line for conditional gene targeting in distinct mono- and multiciliated tissues. Differentiation. 2012 Feb;83(2):S105-13
• All: 5 reference(s)