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Sirt1tm2.1Mcby
Targeted Allele Detail
Nomenclature
Symbol: Sirt1tm2.1Mcby
Name: sirtuin 1; targeted mutation 2.1, Michael W McBurney
MGI ID: MGI:5306956
Synonyms: sirt1Y
Gene: Sirt1  Location: Chr10:63319005-63339035 bp, - strand  Genetic Position: Chr10, 32.57 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:180628
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Hypomorph, Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsA C to T mutation resulting in a histidine to tyrosine substitution at codon 355 (H355Y) was introduced in exon 5 and a floxed hygromycin/thymidine kinase fusion gene with splice acceptor and donor sequences was inserted upstream of exon 5 via homologous recombination. Cre mediated recombination removed the hygromycin/thymidine kinase fusion gene. The mutant protein is expressed at levels similar to the wild-type protein. The mutant protein lacks acetylation activity in MEFs. (J:180628)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 11 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sirt1 Mutation:  51 strains or lines available
References
Original:  J:180628 Seifert EL, et al., SirT1 catalytic activity is required for male fertility and metabolic homeostasis in mice. FASEB J. 2012 Feb;26(2):555-66
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory