Notch3^{tm1.1(cre/ERT2)Sat}
Targeted Allele Detail

Summary

• Symbol: Notch3^{tm1.1(cre/ERT2)Sat}
• Name: notch 3; targeted mutation 1.1, Spyros Artavanis-Tsakonas
• MGI ID: MGI:5304914
• Synonyms: N3-CreERT2^SAT
• Gene: Notch3 Location: Chr17:32339794-32385826 bp, - strand Genetic Position: Chr17, 17.37 cM
• Alliance: Notch3^{tm1.1(cre/ERT2)Sat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179632
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Notch3^{tm1.1(cre/ERT2)Sat} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Notch3 (MGI:99460)

• Mutation details: A targeting vector was constructed to insert the cre/ERT2 coding sequence, a polyadenylation signal and an Frt-flanked neo selection cassette in-frame with the start codon of Notch 3, by homologous recombination in ES cells. This resulted in deletion of most of exon 1. Chimeric males were bred to Flpe-expressing females to remove the selection cassette. (J:179632)

Generation of the various Notch knock-in mice

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Notch3 (mouse) Summary of all recombinase alleles driven by Notch3. Alliance mouse Notch3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch3 Mutation: 93 strains or lines available

References

• Original: J:179632 Fre S, et al., Notch lineages and activity in intestinal stem cells determined by a new set of knock-in mice. PLoS One. 2011;6(10):e25785
• All: 4 reference(s)